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2 edition of Steam pretreatment of wood in relation to enzymatic hydrolysis found in the catalog.

Steam pretreatment of wood in relation to enzymatic hydrolysis

H. H. Brownell

Steam pretreatment of wood in relation to enzymatic hydrolysis

final report

by H. H. Brownell

  • 53 Want to read
  • 18 Currently reading

Published by Forintek Canada Corp. in Ottawa .
Written in English

    Subjects:
  • Wood -- Chemistry

  • Edition Notes

    Includes bibliographical references.

    Statementprepared by H.H. Brownell.
    Classifications
    LC ClassificationsTS936 B76 1988
    The Physical Object
    Paginationxii, 146, 16, 3, 2 leaves. :
    Number of Pages146
    ID Numbers
    Open LibraryOL17194784M

    associated with pretreatment and enzyme usage. Several activities being conducted utilize the capabilities of the Biomass Surface Characterization Laboratory in order to better understand compositional and structural impacts of pretreatment and enzymatic hydrolysis across various pretreatment chemistries and feedstocks. Specific activities in the. This study investigated the potential use of alkali pretreatment of sawdust from Australian timber mills to produce bioethanol. Sawdust was treated using 3–10% w/w NaOH at temperatures of 60, , and −20°C. Two pathways of production were trialled to see the impact on the bioethanol potential, enzymatic hydrolysis for glucose production, and simultaneous saccharification and fermentation Cited by: 3.

    Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder Jun Zhenga, Kim Chooa,b, Chris Bradtc, Rick Lehouxc, Lars Rehmanna,* aDepartment b ofChemical and Biochemical Engineering, University Western Ontario, Richmond Street, London, ON . Table 2 shows the composition of the samples resulting from fungal pretreatment (followed by mild alkali extraction) and a subsequent, or not, acid hydrolysis. Samples that were not subjected to acid hydrolysis exhibited a slight increase in Klason lignin compared to control samples. This was probably due to the removal of other components such as water-soluble extractives and nonstructural Cited by: 2.

    In this experiment, corn stover was treated with optimal combined pretreatment conditions: 2% NaOH at 80 °C treated 2 h combined with initial pH 9 at the ozone concentration of 78 mg/mL treated 25 min. The effect of lignin removal rate on the enzymatic hydrolysis degree of cellulose during the treatment process was studied. At the same time, the lignin in the optimal pretreated corn stover Cited by: 3. The hydrolysis reaction can be effected using strong acids, or enzymes but it is advantageous to employ the enzymatic hydrolysis process because: (1) enzymatic hydrolysis has the potential to give higher glucose yields [15,47], (2) enzymatic hydrolysis can be performed at temperatures only slightly higher than room temperature (50 ± 5°C) [3 Author: Nichola Charles.


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Steam pretreatment of wood in relation to enzymatic hydrolysis by H. H. Brownell Download PDF EPUB FB2

Hydrolysis of steam and acid pretreated wood from eucalyptus by K Pretreatment Severity Enzyme C5 (g/l) C6 (g/l) (log R0) (FPU/g substrate) Total sugar (g/l) Steam Acid steam 2 10 20 2 10 20 Hydrolysis conditions: 10% Cited by:   The most enzymatic hydrolysis studies are conducted at 2–20% total solid to prevent enzyme inhibition.

However, this enzymatic hydrolysis was performed at 25% total solid; the highest sugar yield was achieved from the pretreated LP2 sample with 96% for cellulose and about % for hemicellulose by: Optimization of the steam explosion and enzymatic hydrolysis for sugars production from oak woods the aim of the paper is the optimization of fermentable sugars production for ethanol production from different oak wood residues.

Pretreatment and enzymatic hydrolysis operating parameters were used as process variables in a Response Surface Cited by: 9. UCT-solvent pretreatment was carried out on woods (beech and akamatsu (pine)) for the enzymatic hydrolysis, in which pretreatment the ground woods were autoclaved with a mixture of water and cyclo-hexanol (% vol% cyclohexanol) having upper critical temperature (UCT: °C) on the mutual solubility curve (named as UCT-solvent).

Ninety-five and 92% of Klason lignin were removed Cited by: 6. @article{osti_, title = {Evaluation of pretreatments of biomass for enzymatic hydrolysis of cellulose. [Organosolv process, wet oxidation, and steam explosion of wood chips]}, author = {Chum, H.L.

and Douglas, L.J. and Feinberg, D.A. and Schroeder, H.A.}, abstractNote = {One principal barrier to economic utilization of lignocellulosic feedstocks in enzymatic systems for the production. Steam refi ning of non-debarked poplar wood with SO 2 impregnation prior to steaming was investigated as pretreatment for enzymatic hydrolysis.

Pretreatment conditions were varied in the range of. Therefore, alkaline pretreatment, instead of diluted acid pretreatment, was applied before enzymatic hydrolysis. Alkaline pretreatment with the use of NaOH was reported as an effective method to.

Aspenwood, wheat straw, wheat chaff and alfalfa stems were treated under pressure with either steam or ammonia. The material was then water or methanol/water extracted. The extent of enzymatic hydrolysis of the cellulose portion of the treated substrates was compared using two different cellulases, a commercial preparation, Celluclast, and those from the fungus Trichoderma by: @article{osti_, title = {Similarities and differences in pretreating woody biomass by steam explosion, wet oxidation, autohydrolysis, and rapid steam hydrolysis/continuous extraction}, author = {Schultz, T.P.

and McGinnis, G.D. and Biermann, C.J.}, abstractNote = {Steam hydrolysis processes are excellent methods for pretreating and fractionating cellulose from the other major wood. Richard P. Chandra, QiuLu Chu, Jinguang Hu, Na Zhong, Mandy Lin, Jin-Suk Lee and Jack Saddler, The influence of lignin on steam pretreatment and mechanical pulping of poplar to achieve high sugar recovery and ease of enzymatic hydrolysis, Bioresource Technology,(), ().

lulose to glucose conversion during enzymatic hydrolysis. Rivers and Emert () concluded that particle size of substrates derived from ball milling had no effect on hydrolysis yield in the wet particle size range of mm studied.

Cullis et al. () and Ballesteros et al. () reported that initial wood chip size can affect cellu-Cited by: Raw material and pretreatment. Chips (2 to 10 mm) from debarked Norway spruce (Picea abies) were provided by Witskövfle Sågverk AB, chips were impregnated with % SO 2 (based on moisture content) for 20 minutes in plastic bags.

The material was then steam-pretreated for 5 minutes at °C in a 10 litre reactor, as previously described by Palmqvist et al. [].Cited by: TR-2 I83 SUMMARY Objective I' To examine the theoretical (scientific-based) and practical (engineering and economic- based) constraints to the pretreatment of lignocellulosic biomass in the enzymatic 'I hydrolysis of cellulose based on available pretreatment data.

Discussion ' The pretreatment of biomass is a critical step in the enzymatic hydrolysis of cellulose. Pretreatment and Process Hydrolysis WBS Melvin Tucker National Renewable Energy Laboratory.

This presentation does not contain any proprietary, confidential, or otherwise restricted information. DOE Bioenergy Technologies Office (BETO) Project Peer Review.

March 9, Feedstock-Conversion Interface Consortium. Three strains of Trichoderma—T. reesei C30, T. reesei QM, and Trichoderma species E‐58—were used to study the enzymatic hydrolysis of pretreated wood substrates.

ach of the culture filtrates was incubated with a variety of commercially prepared cellulose substrates and pretreated wood substrates. Solka floc was the most easily degraded commercial cellulose. pretreatment method. Effect of steam explosion temperature on wheat straw enzymatic hydrolysis was investigated.

Optimum steam explosion temperature at around °C was determined based on concentration of monosaccharides in hydrolysates, conversion of cellulose and xylan and yield of monosaccharides from wheat straw.

Preliminary results indicate that attritor milling significantly increases susceptibility of newspaper to enzymatic hydrolysis by J_. viride cellulase. Two-roll milling of newspaper, cotton, and wood chips has been shown effective in enhancing susceptibility to enzymatic hydrolysis.

This was accomplished using 3, 6, and inch diameter mills. Improvement of the process of cellulase production and development of more efficient lignocellulose-degrading enzymes are necessary in order to reduce the cost of enzymes required in the biomass-to-bioethanol process.

Lignocellulolytic enzyme complexes were produced by the mutant Trichoderma atroviride TUB F on three different steam-pretreated lignocellulosic substrates, Cited by: Steam pretreatment in a batch reactor involves heating wood chips at high temperatures and pressures, followed by mechanical disruption of the pretreated material either by violent discharge into a collecting tank (explosion)or by mild blending after bleeding the steam pressure down to atmospheric (no explosion) pretreatment and enzymatic hydrolysis of sorghum bran involved hot water treatment (10% solid, w/v) at °C for 20 min, acid hydrolysis (H 2SO 4), starch degradation, and enzymatic hydrolysis (60 hr, 50°C, %, v/v) with commercial cellulase and hemicellulose enzymes.

Total sugar yield by using enzymatic hydrolysis alone was 9%, obtained from. Liu et al. (). “Kinetics of enzymatic hydrolysis,” BioResources 6(4), Enzymatic Hydrolysis Process A conical flask of mL was selected for the enzymatic hydrolysis and placed in thermostatic cultivation.

The concentration of solid for hydrolysis File Size: KB.However, the cellulose-to-glucose conversion yield of bamboo substrates after direct (without steam explosion pretreatment) sodium chlorite/acetic acid delignification was %.

Fermentation of enzymatic hydrolyzates with Saccharomyces cerevisiae resulted in about % to % of the corresponding theoretical ethanol yield after 24 h.increase in sugar yield after enzymatic hydrolysis. Alkaline (NaOH) is found to be the worst Table Summary of Impact of Pretreatment on Enzymatic Hydrolyis of Softwood Pine.

48 Table Enzymatic Hydrolysis Yield and Enzyme Performance Index of Different Solid'sCited by: 1.